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KMID : 0381920070370040249
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Korean Journal of Microscopy
2007 Volume.37 No. 4 p.249 ~ p.258
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Ultrastructural Change and Insulin Distribution of the Cultured Pancreatic Islet β-cell
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Min Byoung-Hoon
Kim Soo-Jin
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Abstract
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The Pancreatic islet are the clusters of endocrine cells scattered through out the exocrine pancreas. Transplantation of a sufficient pancreatic islets can normalize blood glucose level so that may prevent devastating complications of type I diabetes (IDDM) and other side effects of the IDDM.
Recently, there are several approaches to transplant sufficient pancreatic islet, and it was comprised in increase or regeneration of the endogenous β-cell mass from donor’s pancreas, but relatively few studies have been devoted to the morphological characters of the isolated and 3day cultured pancreatic islets.
We investigated morphological pattern of intracellular structure of isolated and 3day cultured pancreatic islets. The morphological characters of the pancreatic islets were observed by scanning electron microscope and transmission electron microscope, and insulin distribution of the each islets were observed by transmission electron microscope, and were labeled with insulin antibody. Intracellular structures including nuclei, mitochondria, RER, Golgi complex and many secretory granules were normally appeared in the isolated pancreatic islets which was extracted immediately dornor’s pancreas, however, There is a significant morphological changes between the 3 day cultured pancreatic islets and isolated islets. 3 day cultured pancreatic islet’s β-cells had normal nuclei but increased cytoplasm mass and RER and developed Golgi complex. Insulin secretory granules were decreased in numbers rather than isolated pancreatic islet.
In this study, the pattern of intracellular structure variation was examined during pancreatic islet culture. Most distinct features are variation of the insulin secretory granules, and developed RER, and dilated golgi complex. Therefore, we suggested that the various change of the morphological characters on cultured pancreatic islets were responsible for the function (biosynthesis and secretion of insulin) and growth. These results were also cultured islets have greater ability to recover and maintain normoglycemia than isolated islet transplantation.
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KEYWORD
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Cultured pancreatic islet, Insulin, Immuno gold labeling
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