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KMID : 0382419990250040001
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Korean Journal of Environmental Health Society
1999 Volume.25 No. 4 p.1 ~ p.6
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A Study on the Induction of Apoptosis by Cadmium in HL-60 Cells
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Abstract
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Apoptosis is a process of active cell death, distinct from necrosis and characterized by specific morphological and biochemical features. Apoptosis induced by cadmium and cadmium-related deleterious conditions has only recently been studied. Information about cadmium-induced apoptosis is therefore very limited. Although the toxic effects of cadmium are well described, little is known about the mechanism of apoptosis by cadmium toxicity. This study is designed to define the induction of apoptosis by which cadmium exert the cytotoxic effect on human promyelocytic leukemic HL-60 cells. Cadmium (125μM) induce the apoptosis of HL-60 cells which is characterized by the ladder pattern of DNA fragmentation. Cadmium induce the activation of DEVD-specific cysteine protease (caspase-3) in a time dependent manner. They also increase the phosphotransferase activities of c-JUN N-terminal kinase (JNK) in cadmium treated HL-60 cells. Furthermore, cadmium increase the activation of transcriptional factors including activating protein-1 (AP-1) and NF-kB. These results suggest that cadmium induce the apoptotic death of HL-60 cells via activation of DEVD-specific caspase, JNK and transcriptional factors such as AP-1 and NF-kB.
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KEYWORD
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