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KMID : 0385219960060020033
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Korean Journal of Gerontology
1996 Volume.6 No. 2 p.33 ~ p.42
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Hormonal Influence on Tissue Transglutaminase in Rat Prostate [Effect of Orchiectomy and Testosterone]
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Moon Jae-Whan
Song Kye-Yong
Park Sang-Cheol
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Abstract
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In the present study, the comparative pattern of TGase expression in the process of prostate involution has been investigated by biochemical and immunohistochemical analysis. In the experiment, male Sprague-Dawley adult rats were divided into three groupings : control, orchiectomy, and orchiectomy with testosterone administration. Testosterone was injected sub cutaneously daily from the 7th day after orchiectomy at a dose of 20 ㎎/㎏. The animals were sacrificed serially at 1, 3, 5, 7, 9, 11, and 14th day after orchiectomy. The coagulating gland and ventral and dorsal lobes of the prostate were dissected out. TGase Activity was very weak in the ventral lobe, but strong in the dorsal lobe and coagulating gland. In the ventral lobe, the change of TGase activity was mild, while in the dorsal lobe and coagulating gland, it was marked. After orchiectomy, TGase activity decreased significantly in the coagulating gland and dorsal lobe, but returned to the normal level by testosterone treatment. Change in amounts of TGase were very similar to those in TGase activities. In the ventral lobe, the expression pattern of TGase C illustrated the partial sensitivity of its expression to androgen. These results indicate that the actual pattern of TGase C expression in the process of prostate involution may be opposite to the previously held view that its expression might increase during the process of apoptosis. With the concomitant morphological changes during the prostate involution such as the decrease of column heights and the atrophy of glandular structures and increase of fibrosis, etc., the expression of TGase C in those tissues showed the similar changes to those shown by biochemical analysis. From these results, it can be concluded that the expression of TGase is related to cell survival, rather than to apoptosis, programmed cell death, contrary to the previous reports.
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KEYWORD
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Prostate, orchiectomy, testosterone, transglutaminase
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