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KMID : 0385220010110020028
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Korean Journal of Gerontology
2001 Volume.11 No. 2 p.28 ~ p.35
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Involvement of p38 Mitogen-Activated Protein Kinase on Proliferation of Mast Cells
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Chung Jong-Gab
Kim Chang-Young
Hong Seung-Heon
Kim Hyung-Min
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Abstract
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Activation of phosphatidylinositol 3-kinase (PI3-kinase) has been known to be important in mitogenic signal transduction. The function of PI3-kinase in mast cells proliferation remains to be elucidated. Here we demonstrated that in mast cells, PI3-kinase inhibitors, wortmannin and LY294002, decreased the proliferation of mast cells. To identify signalling pathways, we investigated the phosphorylation of p38 mitogen activated protein kinase (MAPK). Selective inhibition of this kinase with LY294002 indicated that p38 MAPK contributed to the proliferation of mast cells. The use of different kinase inhibitors indicated that 5B203580 also inhibited the proliferation of mast cells. Wortmannin treatment of mast cells was followed by transient expression of the mRNA of c-fos and a sustained expression of the mRNA of c-jun. Furthermore, the expression of c-kit receptor decreased in the mast cells treated with wortmannin whereas it was not changed in the cells treated with genistein. These results suggest that PI3-kinase plays an important role in proliferation through p38 in mast cells.
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KEYWORD
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phosphatidylinositol 3-kinase, mast cells, P38 mitogen activated protein kinase, c-fos, c-jun, c-kit receptor
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