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KMID : 0385220040140020024
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Korean Journal of Gerontology
2004 Volume.14 No. 2 p.24 ~ p.30
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Anti-aging Activity of Soybean Isoflavones
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Abstract
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To test in vitro anti-aging activity of isoflavones, a human embryonic fibroblast line that was reported to be grown up to 70 population doubling (PD) was used. The fibroblast line was cultivated up to 50 PD at regular culture medium and changed to isoflavones-containing medium until cells reached replicative senescence. Maximum PD and percent of SA-beta-gal positive staining were measured and compared with untreated cell. Daidzein extended the maximum PD by about 7% and lowered the percent of SA-beta-gal positive staining by 20% while genistein extended the maximum PD by about 4% and lowered the percent of SA-beta-gal positive staining by 15% at 6.7 mg/ml . Two isoflavones showed apparently anti-aging activity in human fibroblast cultre. To confirm in vivo anti-aging activity, 12 month old rats were fed with isoflavone-containing water for 5 month. Either daidzein or genistein-fed rats maintained their body wetght and showed surprisingly a little increase in total activity whereas untreated rats showed gradual decrease in body weight and total activity. The result suggests that anti-aging activity was also apparent in vivo.
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KEYWORD
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Isoflavones, daidzein, genistein, anti-aging
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