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KMID : 0390320030130020151
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Chungbuk Medical Journal
2003 Volume.13 No. 2 p.151 ~ p.166
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Hypermethylation of E-cadherin Gene Promoter in Gastric Cancer Tissues
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Park Mi-Kyung
Kim Heon
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Abstract
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Purpose: Gastric cancer is the most common cancer in Korea. Hypermethylation of E-cadherin gene promoter is found in more than 50% of human gastric cancer tissues, and has been reported to be associated with the decreased expression of E-cadherin. The possibility of invasiveness and metastasis of gastric cancer increases in cases with the decreased expression of E-cadherin. The aim of this study is to investigate the effects of diet and genetic polymorphisms of IL-1β and GSTP1, and their interactions on the hypermethylation of E-cadherin gene promoter in gastric cancer tissues.
Materials and Methods: One hundred and ten gastric cancer patients, and 220 age-and sex-matched controls were enrolled in this study. Direct interview using a semi-quantitative food frequency questionnaire (FFQ) was performed to get informations on the level of exposure to various diet. PCR-RFLP methods were used for the genotyping of IL-1β and GSTP1, and the methylation-specific PCR was applied to check hypermethylation of E-cadherin gene promoter in stomach cancer tissues.
Results: Hypermethylation of E-cadherin gene promoter was detected in 40.0% of gastric cancer tissues. Pork, pettitoes, and pork cutlet were protective factors, whereas kimchi, kimchi stew, and mugwort were risk factors for the hypermethylation of E-cadherin gene promoter· in stomach cancer tissues. However, the hypermethylation of E-cadherin gene promoter was not associated with the genetic polymorphisms of IL-1β or GSTP1.
Conclusion: These results suggest that the hypermethylation of E-cadherin promoter is associated with dietary factors but not with the genetic polymorphisms of IL-1β and GSTP1, and that progression of gastric cancer could be different according to the food intake pattern before the diagnosis of gastric cancer.
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KEYWORD
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Gastric cancer, Genetic polymorphism, E-cadhein, Diet, IL-1β, GSTP1
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