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KMID : 0391319960060010069
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Korean Journal of Biological Response Modifiers
1996 Volume.6 No. 1 p.69 ~ p.77
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A mouse Model for the Study of Biological Functions of IL-2 Induced No Synthesis
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Yim Chang-Yeol
Hwang Tai-Ju
Kim Hyeoung-Joon
Kwak Jae-Yong
Sohn Myung-Hee
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Abstract
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It has been unclear whether IL-2-induced NO synthesis represents either an anticancer effector or a side effect of IL-2 therapy. The purpose of this study is to develop a mouse model to study biologic effects of NO, and the relationship between NO and either anticancer effects or toxicities of IL-2 therapy. We were trying to develop a model having following characteristics. ① IL-2 therapy should delay tumor progression, and ② induce high output NO synthesis. ③ IL-2-induced NO synthesis should be efficiently inhibited by an adequate administration of NG-monomethyl-L-arginine(MLA). ④ A reproducible method for the measurement of the changes in in vivo NO synthesis should be developed. Subcutaneously tumor(RD-995) bearing C3H/HeN mice receiving IL-2(180,000 IU bid i.p. for 5days) were given MLA(0.2 μ 1 of 3.38M) using subcutaneous infusion pumps, and then urine nitrate excretion as a reflection of in vivo NO synthesis, and tumor size were measured daily. The model described in the present study had all the the above characteristics. This model is valuable to study the relationship between NO and either anticancer effects or toxicities of IL-2 therapy, and may also be applicable to study biologic effects of other cytokine therapies including interferons, tumor necrosis factor, IL-1, IL-6, and colony stimulating factors which were known to be related to NO production.
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KEYWORD
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Nitric Oxide, Interleukin-2, Tumor, Osmotic, Minipump, L-arginine
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