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KMID : 0425119960340030211
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Parasites, Hosts and Diseases
1996 Volume.34 No. 3 p.211 ~ p.223
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Electrophoretic variations of enzyme, GDH(NADP-dependent glutamate dehydrogenase)(EC. 1.4.1.4) in characterizing clones and isolates of Malaysian Plasmodium falciparum (Brief Communication)
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Hooi Hoon ANG
Kit Lam CHAN/Joon Wah MAK
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Abstract
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Malaysian, African and Thai Plasmodium falciparum isolates were cultured in vitro by the Trager and Jensen method (1976; 1977) and were later cloned by the limiting dilution method (Rosario, 1981). Forty- eight clones were obtained and were characterized by electrophoretic variations of GDH (NADP-dependent glutamate dehydrogenase)(EC. 1.4.1.4). It was found that they were pure clones because they possessed either GDH-1 or GDH-2 unlike their parent isolates which exhibited both GDH-1 and GDH-2.
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KEYWORD
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