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KMID : 0425119990370010059
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Parasites, Hosts and Diseases
1999 Volume.37 No. 1 p.59 ~ p.62
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Characterization of YS-27, an axenic Korean strain of Entamoeba histolytica
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Soon Jung PARK
Tai Soon YONG/Jae Kyung CHANG/Kyung Il IM/Chin Thack SOH/Soon Jung PARK/Tai Soon YONG/Jae Kyung CHANG/Kyung Il IM
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Abstract
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Characterization of YS-27, an axenic Entamoeba strain, was performed by three different laboratory methods. Zymodeme analysis using starch gel electrophoresis and PCR with species-specific primers showed that YS-27 is a pathogenic Entamoeba which belongs to the group II zymodeme. Pathogenicity of YS-27 was further confirmed by observing the formation of liver abscess in Mongolian gerbils. These results showed that YS-27 is E. hisolytica.
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KEYWORD
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Entamoeba histolytica, zymodeme, PCR, liver abscess,
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