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KMID : 0425120000380030195
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Parasites, Hosts and Diseases
2000 Volume.38 No. 3 p.195 ~ p.199
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Differential expression of the 27 kDa cathepsin L-like cysteine protease in developmental stage of Spirometra erinacei
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Shin-Yong KANG/Yoon KONG
Doo-Hee YUN/Seung-Yull CHO/Woon-Mok SOHN*/Young-Bae CHUNG†/Shin-Yong KANG†
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Abstract
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The 27 kDa cathepsin L-like cysteine protease of Spirometra erinacei plerocercoid is known to play an important function in tissue penetration, nutrient uptake and immune modulation in human sparganosis. In the present study, the expression of this enzyme was examined at different developmental stages of S. erinacei including immature egg, coracidium, plerocercoid in tadpole and rat, and adult. Proteolytic activity against carboxybenzoyl-phenylalanyl-arginyl-7-amino-4-methylcoumarin was detected in the extracts of coracidia and plerocercoid while no activity was observed in those of immature egg and adult. The specific activity in coracidial extracts was lower than that in the plerocercoid. Reverse transcription-polymerase chain reaction and Northern blot analysis demonstrated that the gene was expressed in the coracidium and plerocercoid but not in immature egg and adult. These results suggest that the 27 kDa cysteine protease is only expressed in the stages involving active migration of the parasite in the host tissue.
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KEYWORD
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Spirometra erinacei, Cysteine protease, developmental expression,
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