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KMID : 0425120010390020151
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Parasites, Hosts and Diseases
2001 Volume.39 No. 2 p.151 ~ p.160
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Expressed sequence tags (ESTs) analysis of Acanthamoeba healyi
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Dong Il Chung/Hyun Hee Kong
Mee Yeul Hwang/Hyo Kyung Kim/Dong Il Chung
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Abstract
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Randomly selected 435 clones from Acanthamoeba healyi cDNA library were sequenced and a total of 387 expressed sequence tags (ESTs) had been generated. Based on the results of BLAST search, 130 clones (34.4%) were identified as the genes enconding surface proteins, enzymes for DNA, energy production or other metabolism, kinases and phosphatases, protease, proteins for signal transduction, structural and cytoskeletal proteins, cell cycle related proteins, transcription factors, transcription and translational machineries, and transporter proteins. Most of the genes (88.5%) are newly identified in the genus Acanthamoeba. Although 15 clones matched the genes of Acanthamoeba located in the public databases, twelve clones were actin gene which was the most frequently expressed gene in this study. These ESTs of Acanthamoeba would give valuable information to study the organism as a model system for biological investigations such as cytoskeleton or cell movement, signal transduction, transcriptional and translational regulations. These results would also provide clues to elucidate factors for pathogenesis in human granulomatous amoebic encephalitis or keratitis by Acanthamoeba.
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KEYWORD
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Acanthamoeba healyi, expressed sequence tags, novel genes,
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