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KMID : 0425120070450030229
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Parasites, Hosts and Diseases
2007 Volume.45 No. 3 p.229 ~ p.232
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Metacercarial proteins interacting with WD40-repeat protein of Clonorchis sinensis
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Cho Pyo-Yun
Kim Tae-Im
Li Shun-Yu
Hong Sung-Jong
Choi Min-Ho
Hong Sung-Tae
Chung Yong-Je
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Abstract
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The WD40-repeat proteins serve as a platform coordinating partner proteins and are involved in a range of regulatory cellular functions. A WD40-repeat protein (CsWD1) of Clonorchis sinensis previously cloned is expressed stage-specifically in the tegumental syncytium of C. sinensis metacercariae. In the present study, interacting proteins with the CsWD1 protein was purified by immunoprecipitation and 2 dimension gel electrophoresis from the C. sinensis metacercaria soluble extract, and tryptic peptides were analyzed by LC/ESI-MS. Putative partner proteins were annotated to be actin-2, glyceraldehyde-3-phosphate dehydrogenase, and hypothetical and unmanned proteins. The CsWD1 protein was predicted to contain 3 conserved actin-interacting residues on its functional surface. With these results, the CsWD1 protein is suggested to be an actin-interacting protein of C. sinensis
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KEYWORD
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Clonorchis sinensis, WD40-repeat protein, actin
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