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KMID : 0425120090470030287
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Parasites, Hosts and Diseases
2009 Volume.47 No. 3 p.287 ~ p.291
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Evaluation of α-Tubulin as an Antigenic and Molecular Probe to Detect Giardia lamblia
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Kim Ju-Ri
Park Soon-Jung
Song Kyoung-Ju
Shin Myeong-Heon
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Abstract
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The α/β-tubulin heterodimer is the basic subunit of microtubules in eukaryotes. Polyclonal antibodies specific to recombinant α-tubulin of Giardia lamblia were made, and found effective as a probe to specifically detect G. lamblia by immunofluorescence assays. Nucleotide sequences of α-tubulin genes were compared between G. lamblia WB and GS strains, prototypes of assemblage A and assemblage B, respectively. A set of primers was designed and used to amplify a portion of the α-tubulin gene from G. lamblia. PCR-RFLP analysis of this α-tubulin PCR product successfully differentiated G. lamblia into 2 distinct groups, assemblages A and B. The results indicate that α-tubulin can be used as a molecular probe to detect G. lamblia.
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KEYWORD
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Giardia lamblia, α-tubulin, immunofluorescence assay, PCR-RFLP
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