|
KMID : 0425120100480040343
|
|
Parasites, Hosts and Diseases
2010 Volume.48 No. 4 p.343 ~ p.346
|
|
|
Prevalence of Haplorchis taichui in Field-Collected Snails: A Molecular Approach
|
|
Chontananarth Thapana
Wongsawad Chalobol
|
|
|
Abstract
|
|
|
|
The prevalence of the cercarial stage of an intestinal trematode, Haplorchis taichui, in thiarid snails (Gastropoda: Thiaridae) was investigated using light microscope and species-specific PCR procedures. A total of 988 snails were collected from Mae Taeng district, Chiang Mai province, northern Thailand, which comprised of 3 species; Melanoides tuberculata,Tarebia granifera, and Thiara scabra. The overall prevalence of pleurolophocercous cercariae was 21.7% as determined by the morphology. For genetic detection of H. taichui infection in snails, 2 primers Hapt_F (5’-GGCCAACGCAATCGTCATCC-3’) and Hapt_R (5’-GCGTCGGGTTTCAGACATGG-3’), were used. The genomic DNA of H. taichui, which was used as a positive control, gave an amplification of the 256 bp fragment. The overall prevalence of H. taichui from specific PCR was 9.7%. The proportion of H. taichui among the pleurolophocercous cercariae in this study was 44.9%.
|
|
|
KEYWORD
|
|
|
Haplorchis taichui, pleurolophocercous cercaria, thiarid snail, PCR, Thailand
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
    
|
|