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KMID : 0425120110490010085
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Parasites, Hosts and Diseases
2011 Volume.49 No. 1 p.85 ~ p.90
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CD8+ T-cell Activation in Mice Injected with a Plasmid DNA Vaccine Encoding AMA-1 of the Reemerging Korean Plasmodium vivax
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Kim Hyo-Jin
Jung Bong-Kwang
Lee Jin-Joo
Pyo Kyoung-Ho
Kim Tae-Yun
Choi Byung-Il
Kim Tae-Woo
Hisaeda Hajime
Himeno Kunisuke
Shin Eun-Hee
Chai Jong-Yil
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Abstract
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Relatively little has been studied on the AMA-1 vaccine against Plasmodium vivax and on the plasmid DNA vaccine encoding P. vivax AMA-1 (PvAMA-1). In the present study, a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax has been constructed and a preliminary study was done on its cellular immunogenicity to recipient BALB/c mice. The PvAMA-1 gene was cloned and expressed in the plasmid vector UBpcAMA-1, and a protein band of approximately 56.8 kDa was obtained from the transfected COS7 cells. BALB/c mice were immunized intramuscularly or using a gene gun 4 times with the vaccine, and the proportions of splenic T-cell subsets were examined by fluorocytometry at week 2 after the last injection. The spleen cells from intramuscularly injected mice revealed no significant changes in the proportions of CD8+ T-cells and CD4+ T-cells. However, in mice immunized using a gene gun, significantly higher (P<0.05) proportions of CD8+ cells were observed compared to UB vector-injected control mice. The results indicated that cellular immunogenicity of the plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax was weak when it was injected intramuscularly; however, a promising effect was observed using the gene gun injection technique.
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KEYWORD
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Plasmodium vivax, DNA vaccine, apical membrane antigen (AMA), CD8+ T-cell, gene gun
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