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KMID : 0425120110490030281
Parasites, Hosts and Diseases
2011 Volume.49 No. 3 p.281 ~ p.284
PCR Diagnosis of Entamoeba histolytica Cysts in Stool Samples
Moon Joung-Ho

Cho Shin-Hyeong
Yu Jae-Ran
Lee Won-Ja
Cheun Hyeng-Il
Abstract
Amebiasis is a protozoan disease caused by Entamoeba histolytica and a potential health threat in areas where sanitation and hygiene are inappropriate. Highly sensitive PCR methods for detection of E. histolytica in clinical and environmental samples are extremely useful to control amebiasis and to promote public health. The present study compared several primer sets for small subunit (SSU) rDNA and histone genes of E. histolytica cysts. A 246 bp of the SSU rDNA gene of pure cysts contained in phosphate-buffered saline (PBS) and in stool samples was successfully amplified by nested PCR, using the 1,147-246 bp primer set, of the primary PCR products which were pre-amplified using the 1,147 bp primer as the template. The detection limit of the nested PCR using the 1,147-246 primer set was 10 cysts in both groups (PBS and stool samples). The PCR to detect histone gene showed negative results. We propose that the nested PCR technique to detect SSU rDNA can be used as a highly sensitive genetic method to detect E. histolytica cysts in stool samples.
KEYWORD
Entamoeba histolytica, SSU rDNA, PCR, diagnosis, stool
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