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KMID : 0425120190570010061
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Parasites, Hosts and Diseases
2019 Volume.57 No. 1 p.61 ~ p.67
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Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep
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Liu Tianli
Wang Xifeng
Tian Zhenzhong
Wang Lixia
Zhang Xingxing
Qiao Jun
Meng Qingling
Gong Shasha
Chen Ying
Cai Xuepeng
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Abstract
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Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.
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KEYWORD
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multi-epitope, fusion protein, serodiagnostic, antigen, cystic echinococcosis, indirect ELISA, sheep
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