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KMID : 0425120190570030309
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Parasites, Hosts and Diseases
2019 Volume.57 No. 3 p.309 ~ p.312
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Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China
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He Li
Fang Zheng-Ming
Xue Ting
Zhang Er-Fu
An Chun-Li
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Abstract
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Spargana were collected from human and frogs in Liaoning and Hubei Provinces, China. PCR amplification and direct sequencing of A cox1 fragment was PCR-amplified from genomic DNA extracted from 7 specimens (5 from humans and 2 from frogs). The cox1 fragment (390 bp) showed 97-100% similarity to the reference sequence of S. erinaceieuropaei and 88-89% to the reference sequence of S. decipiens. There were 1-12 bases different between these worms, but no obvious genetic variation (0-3.3%) to the references. There was little difference of cox1 gene between sparganum samples of humans and frogs (1-3%). This study is the first report on S. erinaceieuropaei spargana from humans in Liaoning and Hubei Provinces.
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KEYWORD
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Spirometra erinaceieuropaei, spargana, cox1, genetic analysis
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