KMID : 0425120200580060675
|
|
Parasites, Hosts and Diseases 2020 Volume.58 No. 6 p.675 ~ p.679
|
|
Functional Identification of a Nuclear Localization Signal of MYB2 Protein in Giardia lamblia
|
|
Kim Ju-Ri
Shin Mee-Young Park Soon-Jung
|
|
Abstract
|
|
|
MYB2 protein was identified as a transcription factor that showed encystation-induced expression in Giardia lamblia. Although nuclear import is essential for the functioning of a transcription factor, an evident nuclear localization signal (NLS) of G. lamblia MYB2 (GlMYB2) has not been defined. Based on putative GlMYB2 NLSs predicted by 2 programs, a series of plasmids expressing hemagglutinin (HA)-tagged GlMYB2 from the promoter of G. lamblia glutamate dehydrogenase were constructed and transfected into Giardia trophozoites. Immunofluorescence assays using anti-HA antibodies indicated that GlMYB2 amino acid sequence #507?#530 was required for the nuclear localization of GlMYB2, and this sequence was named as NLSGlMYB2. We further verified this finding by demonstrating the nuclear location of a protein obtained by the fusion of NLSGlMYB2 and G. lamblia glyceraldehyde 3-phosphate dehydrogenase, a non-nuclear protein. Our data on GlMYB2 will expand our understanding on NLSs functioning in G. lamblia.
|
|
KEYWORD
|
|
Giardia lamblia, nuclear localization signal, GlMYB2
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|