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KMID : 0545119910010040256
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Journal of Microbiology and Biotechnology
1991 Volume.1 No. 4 p.256 ~ p.260
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The Regulation of Alpha-Amylase Synthesis in Bacillus subtilis
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WON, MISUN
CHAMBLISS, GLENN H./SONG, KYUNG BIN
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Abstract
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In B, subtilis, α-amylase synthesis is regulated by amyR located directly on the upstream of amyE. Three different amyR alleles have been reported, amyRl, amyR2 and amyR3. Strains bearing the gra-10 mutation which confers derepression for catabolite repression has G→A transition mutation at +5 of amyR1. S1 nuclease mapping demonstrated that transcription initiated at 8 bases downstream from the -10 region of putative Eσ^A promoter P1 in amyR1 and gra-10. In amyR2, the major transcription initiatd at the same place and the minor, 10 bases downstream from -10 of P2. The transcript from P2 contributed approximately 15-20% of total amyE mRNA. S1 nuclease protection experiment indicated that amyE mRNA levels corresponded to the rate of synthesis assumed by specific activities of α-amylase in culture supernatants, suggesting that α-amylase synthesis is regulated at the level of transcription.
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