|
KMID : 0545119920020010046
|
|
Journal of Microbiology and Biotechnology
1992 Volume.2 No. 1 p.46 ~ p.49
|
|
|
Construction of Astaxanthin Overoproducing Strain of Phaffia rhdozyma by protoplast Fusion
|
|
KOH, MOO SUK
KIM, SANG MOON/CHUN, SOON BAI
|
|
|
Abstract
|
|
|
|
The availability of Phaffia rhodozyma as an astaxanthin sources in the aquaculture industry is limited because of the low carotenoid content of natural isolate. In this study, we have used the protoplast fusion technique to construct cell hybrids with an increased content of astaxanthin from P. rhodozyma. Cell hybrids (F307 and F406) obtained were very stable and produced considerably more astaxanthin (>1㎎/g yeast) than the wild parent Karyogamy was confirmed by the isolation of recombinants after mitotic segregation of parental auxotrophic genetic markers, the increased amount of chromosomal DNA/cell and the presence of single nucleus/cell.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|