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KMID : 0545119940040010063
Journal of Microbiology and Biotechnology
1994 Volume.4 No. 1 p.63 ~ p.67
Cometabolism in the Biodegradation of Benzene, Toluene, and p-Xylene Mixture by Isolated Pseudomonas fluorescence BE103
Lim, Hye Kyung
Lee, Jang Young/Kim, Hak Sung
Abstract
A microorganism showing degradative activity towards benzene, toluene and p-xylene (BTX) was isolated from an activated sewage sludge and was tentatively identified as Pseudomonas fluorescence BE103. This strain was found to utilize benzene and toluene as growth substrates, but to degrade p-xylene in the obligate presence of a growth substrate. The metabolic product resulted from the cometabolism of p-xylene was identified as 3,6-dimethylpyrocatechol by LC/MS analysis, and the metabolic pathway was analyzed to be similar to the tod pathway. From the kinetic studies done regarding BTX biodegradation using Pseudomonas fluorescence BE103, it was revealed that the cometabolism of p-xylene is significantly affected by the ratio of growth substrate concentration to biomass concentration, and that the cometabolism of p-xylene initiates only when this ratio was about 0.03.
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