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KMID : 0545119940040030191
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Journal of Microbiology and Biotechnology
1994 Volume.4 No. 3 p.191 ~ p.194
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Effect of Volume Concentration Ratio of Cell-free Medium on Tetramethylpyrazine Production by Lactococcus lactis subsp. lactis biovar. diacetilactis FC1
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Lee, Ji Eun
Woo, Gun Jo/Lee, Hyong Joo
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Abstract
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Acetoin and ammonia, the precursors of tetramethylpyrazine (TMP) having "nutty" or "roasted" flavors, were produced by cultivating Lactococcus lactis ssp. lactis biovar. diacetilactis FC1. The effects of the volume concentration ratio (VCR) of cell-free medium on the formation of TMP were investigated using a rotary evaporator at 70℃ and 80℃. As the VCR increased, the formation of TMP and the conversion ratio of acetoin to TMP increased. More TMP were formed at 70℃ than at 80℃. As the VCR increased, the concentration of acetoin decreased implying the formation of TMP from acetoin and ammonia.
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