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KMID : 0545119940040040285
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Journal of Microbiology and Biotechnology
1994 Volume.4 No. 4 p.285 ~ p.289
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Construction of Recombinant Xanthomonas campestris Strain Producing Insecticidal Protein of Bacillus thuringiensis
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Shin, Byung Sik
Koo, Bon Tag/Choi, Soo Keun/Park, Seung Hwan/Kim, Jeong Il
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Abstract
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An insecticidal crystal protein gene, crylA(c), from Bacillus thuringiensis HD-73 was integrated into the chromosome of a xanthan-producing bacterium, Xanthomonas campestris XP92. The crylA(c) gene expression cassette was constructed that placed the gene between the trc promoter and rmB transcriptional terminator. The lacl^q gene was also included to prevent the expression of crylA(c) gene in X campestris cells. Southern blot analysis confirmed the integration of the crylA(c) gene expression cassette in chromosome of X campestris XP92 transconjugant. Expression of the insecticidal crystal protein was confirmed by Western blot analysis and bioassay against the larvae of Hyphantria cunea (Lepidoptera: Arctiidae) and Plutella xylostella (Lepidoptera: Plutellidae).
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