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KMID : 0545119950050020092
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Journal of Microbiology and Biotechnology
1995 Volume.5 No. 2 p.92 ~ p.95
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Alginate Lyase Production of Halophilic Pseudomonsa sp. by Recombinant Escherichia coli
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KONG, IN SOO
KIM, YOUNG OK/KIM, JIN MAN/KIM, SUNG KOO/OH, DOO HWAN/YU, JU HYUN/KONG, JAI YUL
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Abstract
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Halophilic Pseudomonas sp.W7 isolated from laver in the southern sea of Korea showed alginate lyase activity. Gene (aly) encoding alginate lyase was cloned in E.coli JM83 and the N-terminal amino acid sequence of the enzyme was determined after purificaion. The recombinant enzyme has been shown to have a molecular weight of about 40kDa after 12% SDS-polyacrylamide gel electrophoresis.
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