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KMID : 0545119980080060618
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Journal of Microbiology and Biotechnology
1998 Volume.8 No. 6 p.618 ~ p.624
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Production and Rheological Properties of Bioflocculant Produced by Bacillus sp. DP-152
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Suh, Hyun Hyo
Moon, Seong Hoon/Kim,Hee Sik/Kim, Hyoung Kab/Jun, Gee Ill/Park, Hyun Geoun/Kang, Dae Ook
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Abstract
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The culture conditions for Bacillus sp. DP-152 in the flask were investigated for the production of polysaccharide flocculant, DP-152. The optimum pH and temperature for the flocculant production were 8.0 and 30℃, respectively. The favorable substrates for flocculant production were soluble starch and ammonium nitrate. The medium composition was optimized as follows: 30 g soluble starch, 0.75 g NH_4NO_3, 2.0 g K_2HPO_4,, 0.1 g KH_2PO_4, 0.2 g MgSO_4. 7H_2O, and 0.2 g MnSO_4·4∼5H_2O in 1 l of distilled water. Under this optimized condition, flocculating activity has been improved 4-fold compared with that of the basal medium. In the culture flask, the highest flocculating activity was obtained after 70 h of cultivation and the amount of bioflocculant DP-152 yielded was 12.4 g/l. The solution of bioflocculant DP-152 showed non-Newtonian characteristics. Bioflocculant DP-152 exhibited apparently higher viscosity at all concentrations compared to that of zooglan (from Zoogloea ramigera), and it was stable over a wide range of temperatures and pHs.
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