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KMID : 0545119990090050613
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 5 p.613 ~ p.618
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Isolation of a High-Yield Mutant Strain for L-Proline Production and Its Fermentation Conditions
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Ryu, Wuk Sang
Jang, Hyung Wook/Cho, KYoung Hee/Chang, Soon Jae/Ryu, Yeon Woo/Park, Young Hoon
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Abstract
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L-Proline-producing mutant strains were developed by exposing L-glutamic acid-producing bacteria to Ν-metyl-Ν-nitro-nitrosoguanidine and UV irradiation. A L-histidine auxotroph of Corynebacterium acetoacidophilum RYU3161 (KCTC 0616BP), which was resistant to sulfaguanidine and proline analogs (DHP, AZC, TAC), was isolated. The activity of the mutant strain’s γ-glutamyl kinase was 45% higher than that of the parent strain. The optimum level of L-histidine for production of L-proline was 0.16 g/l. In a 5-1 jar fermenter, the mutant strain produced L-proline at a high concentration (35 g/l) level within 48 h of cultivation.
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KEYWORD
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