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KMID : 0545120000100010016
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 1 p.16 ~ p.20
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Antitumor Activity of Paecilomyces japonica is Mediated by Apoptotic Cell Death
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Park, Youn Hee
Moon, Eun Kyung/Shin, Yong Kyu/Bae, Myung Ae/Kim, Jong Guk/Kim, Young Ho
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Abstract
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The aqueous extract from the cultural mycelium of Paecilomyces japonica showed cytotoxicity against several tumor cells including Jurkat, U937, HL-60, HepG2, BW5147.G.1.4, and NIH3T3. When the aqueous extract was fractionated by sequential organic solvent extractions using nhexane and ethyl acetate, the ethyl acetate fraction appeared to contain the most cytotoxic activity, and the IC_50, values for various tumor cells were in the range from 1.5 to 10.0㎍/㎖. To elucidate the cellular mechanism underlying the induced cytotoxicity, the apoptotic DNA fragmentation along with the cell cycle progression was examined in Jurkat T cells following the ethyl acetate fraction treatment. In the presence of 2.5㎍/㎖ of the ethyl acetate fraction, apoptotic DNA fragmentation of the cells was detected within 1h and increased upto 24h in a time-dependent manner. Under the same conditions, a sub-G1 peak was detectable by flow cytometry. These results indicate that the cytotoxic effect of P japonica on tumor cells is attributable to the induced apoptosis.
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