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KMID : 0545120000100060840
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 6 p.840 ~ p.843
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Expression of the Galactose Mutarotase Gene from Lactococcus lactis ssp.lactis ATCC 7962 in Escherichia coli
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LEE, JONG-HOON
CHOI, JAE YEON/LEE, JUNG MIN/KIM, JEONG HWAN/CHANG, HAE CHOON/CHUNG, DAE KYUN/LEE, HYONG JOO
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Abstract
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The structure of gal/lac operon of Lactococcus lactis ssp. lactis ATCC7962 was partially characterized and the gene (galM) encoding galactose mutarotase was cloned together with the order: galA-galM-galK-galT. The galM was found to be 1,027bp in length and encoded the protein of 37,609Da calculated molecular mass. The deduced amino acid sequence showed a homology with Ga1M proteins from several other microorganisms. Thus, the galM gene was expressed in Escherichia coli and the product was identified as a 38kDa protein which corresponded to the size estimated from DNA sequence. Mutarotase activity of the IPTG induced recombinant was 2.7times increased against that of the host strain.
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KEYWORD
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