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KMID : 0545120020120010149
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 1 p.149 ~ p.152
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Effect of Pressure on Catalytic Properties of Glutamate Racemase from Aquifex pyrophilus, an Extremophilic Bacteria
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LEE, KI SEOG
CHI, YOUNG MIN/YU, YEON GYU
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Abstract
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The effect of pressure on the catalytic properties of glutamate racemase from Aquifex pyrophilus, an extremophilic bacterium, was investigated. The activation volume for the overall reaction (ΔV^?) and catalysis (AV^?_cat) was -96.97 ml/㏖ and 4.97 ml/㏖, respectively, while the reaction volume for the substrate binding (ΔV_K(^-1_m) was -101.94 ml/㏖. The large negative ΔV^? for the overall reaction indicated that the pressurization of glutamate racemase resulted in enhanced catalytic efficiencies. In addition, this value was also due to the large negative ΔV_K(^-1_m) for the substrate binding. The negative value of ΔV_K(^-1_m) implied that the conformational changes in the enzyme molecule occurred during the substrate binding process, thereby increasing the degree of hydration. The small value of ΔV^?_cat suggested that the pressure did not affect the glutamate racemase catalysis after the substrate binding.
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