KMID : 0545120020120030497
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Journal of Microbiology and Biotechnology 2002 Volume.12 No. 3 p.497 ~ p.502
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Diversity of Leuconostocs on Garlic Surface, an Extreme Environment
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Kim, Myung Hee
Shim, Sun Taek/Kim, Youn Soon/Kyung, Kyu Hang
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Abstract
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Thirty-nine strains of Leuconostocs found to be tolerant to 10% or more garlic were selected for further identification, by comparing their whole-cell protein pattern, 16S rRNA gene (first 530 bases) sequence, cellular fatty acid composition, and carbon source metabolism. Two isolates were identified as Leuconostoc mesenteroides and 32 others as Leuconostoc citreum. Five other strains belonging to a cluster could not be allocated to the existing species. 16S rRNA gene sequence and cellular fatty acid composition of the unidentified bacteria exhibited close similarity with Leuconostoc argentinum. The unidentified isolates were not allocated to L. argentinum, because they formed polysaccharide from sucrose, while L. argentinum strains do not. Leuconostocs tolerant to high concentration of garlic were found predominantly on garlic surface, an extreme environment which is unfit for most of other microorganisms.
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