KMID : 0545120030130020313
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Journal of Microbiology and Biotechnology 2003 Volume.13 No. 2 p.313 ~ p.316
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Demonstration of Two Independent Dextranase and Amylase Active Sites on a Single Enzyme Elaborated by Lipomyces starkeyi KSM 22
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LEE, SO-YOUNG
LEE, JIN-HA/ROBYT, JOHN F./SEO, EUN-SEONG/PARK, HYEN-JOUNG/KIM, DOMAN
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Abstract
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Lipomyces starkeyi KSM 22 elaborates an enzyme that has both dextranase and amylase activities in a single protein of 100 kDa. Competition studies, using different amounts of dextran and starch as substrates, gave a competition plot consistent with the hypothesis that the hydrolysis of dextran and starch occurs at two independent active sites, each specific for starch and dextran, respectively.
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KEYWORD
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