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KMID : 0545120030130020317
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Journal of Microbiology and Biotechnology
2003 Volume.13 No. 2 p.317 ~ p.320
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Drug-Induced Haploinsufficiency of Fission Yeast Provides a Powerful Tool for Identification of Drug Targets
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PARK, JO-YOUNG
JANG, YOUNG-JOO/YOU, SEOG-JONG/KIL, YOUNG-SOOK/KANG, EUN-JUNG/AHN, JEE-HEE/RYOO, YOUNG-KWON
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Abstract
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Genome-wide systematic deletion mutants were generated using a PCR-based targeted mutagenesis of Schizosaccharomyces pombe. In a drug-sensitivity assay using thiabendazole (TBZ), an inhibitor of microtubule assembly, a heterozygous nda2 mutant (nda^2+/nda^2-), deleting one copy of nda2 encoding the microtubule subunit alphal demonstrated a distinct sensitivity to TBZ, indicating TBZ-drug-induced haploinsufficiency. This result suggests that profiling drug-induced haploinsufficiency can be exploited to identify target genes for drugs and discover new drugs.
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KEYWORD
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