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KMID : 0545120030130030463
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Journal of Microbiology and Biotechnology
2003 Volume.13 No. 3 p.463 ~ p.468
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Plant Terpenes Enhance Survivability of Polychlorinated Biphenyl (PCB) Degrading Pseudomonas pseudoalcaligenes KF707 Labeled with gfp in Microcosms Contaminated with PCB
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Oh ET
Koh SC/Kim EB/Ahn YH/So JS
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Abstract
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Polychlorinated biphenyls are toxic pollutants and their degradation is quite slow in the environment. Recently interest in bioremediation using PCB-degrading bacteria has increased. In a previous report plant terpenes (p-cymene (S)- (-)-limonene a-pynene and a-terpinene) have been found to be utilized by a PCB degrader and to induce the biphenyl dioxygenase gene in pure culture. In this study Pseudomonas pseudoalcaligenes KF707 a PCB-degrading Gram-negative soil bacterium was used to determine whether the terpene stimulation of PCB degrader occurred in the natural environment. First P. pseudoalcaligenes KF707 was genetically tagged using a transposon with gfp (green fluorescent protein) as a reporter gene. The population dynamics of P. pseudoalcaligenes KF707 harboring gfp gene in a PCB-contaminated environment was examined with or without terpenoids added to the microcosm. About 10-100-fold increase was found in the population of PCB degraders when terpene was added compared with control (non-terpenes samples and biphenyl added samples). It was proposed that the gfp-monitoring system is very useful and terpenes enhance the survivability of PCB degraders in PCB-contaminated environments.
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KEYWORD
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