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KMID : 0545120040140030540
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Journal of Microbiology and Biotechnology
2004 Volume.14 No. 3 p.540 ~ p.546
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Catalytic Oxidoreduction of Pyruvate/Lactate and Acetaldehyde/Ethanol Coupled to Electrochemical Oxidoreduction of NAD(+)/NADH
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Shin IH
Jeon SJ/Park HS/Park DH
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Abstract
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We deviced a new graphite-Mn(II) electrode and found that the modified electrode with Mn(II) can catalyze NADH oxidation and NAD+ reduction coupled to electricity production and consumption as oxidizing agent and reducing power respectively. In fuel cell with graphite-Mn(II) anode and graphite-Fe(III) cathode the electricity of 1.5 coulomb (A x s) was produced from NADH which was electrochemically reduced by the graphite-Mn(II) electrode. When the initial concentrations of pyruvate and acetaldehyde were adjusted to 40 mM and 200mM respectively about 25 mM lactate and 35 mM ethanol were produced from 40 mM pyruvate and 200 mM acetaldehyde respectively by catalysis of ADH and LDH in the electrochemical reactor with NAD+ as cofactor and electricity as reducing power. By using this new electrode with catalytic function the bioelectrocatalysts are engineered; namely oxidoreductase (e.g. lactate dehydrogenase) and NAD+ can function for biotransformation without electron mediator and second oxidoreductase for NAD+/NADH recycling.
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KEYWORD
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