|
KMID : 0545120070170091498
|
|
Journal of Microbiology and Biotechnology
2007 Volume.17 No. 9 p.1498 ~ p.1503
|
|
|
Isolation and Characterization of Strain of Bacillus thuringiensis subsp. kenyae Containing Two Novel cry1-Type Toxin Genes
|
|
Choi Jae-Young
Li Ming-Shun
Shim Hee-Jin
Roh Jong-Yul
Woo Soo-Dong
Jin Byung-Rae
Boo Kyung-Saeng
Je Yeon-Ho
|
|
|
Abstract
|
|
|
|
To identify novel crystal proteins, Bacillus thuringiensis 2385-1 was isolated from Korean soil samples and characterized. The H-serotype of 2385-1 was identical to that of subsp. kenyae (H4a4c), and its crystal toxin was bipyramidal-shaped. However, 2385-1 showed a much higher toxicity towards Plutella xylostella and Spodoptera exigua larvae than subsp. kenyae. In addition, the crystal protein profile and plasmid DNA pattern of 2385-1 differed from those of subsp. kenyae. To verify the crystal protein gene types of 2385-1, a PCR-RFLP analysis was performed, and the results revealed that 2385-1 contained two novel cry1- type crystal protein genes, cry1-5 and cry1-12, in addition to the cry1Ja1 gene. The deduced amino acid sequences of cry1-5 and cry1-12 showed a 97.9% and 75.7% sequence similarity with the Cry1Ab and Cry1Ja crystal proteins, respectively. Among the novel crystal proteins, Cry1-5 showed a high toxicity towards P. xylostella and S. exigua larvae. In conclusion, B. thuringiensis 2385-1 is a new isolate in terms of its gene types, and should be a promising source for an insecticide to control lepidopteran larvae
|
|
|
KEYWORD
|
|
|
Bacillus thuringiensis 2385-1, PCR-RFLP, cry1- type gene, insecticidal activity, novel crystal protein
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|