|
KMID : 0545120080180020355
|
|
Journal of Microbiology and Biotechnology
2008 Volume.18 No. 2 p.355 ~ p.364
|
|
|
Molecular Mechanism of Macrophage Activation by Exopolysaccharides from Liquid Culture of Lentinus edodes
|
|
Lee Ji-Yeon
Kim Joo-Young
Lee Yong-Gyu
Rhee Man-Hee
Hong Eock-Ki
Cho Jae-Youl
|
|
|
Abstract
|
|
|
|
Mushrooms are regarded as one of the well-known foods and biopharmaceutical materials with a great deal of interest. β-Glucan is the major component of mushrooms that displays various biological activities such as antidiabetic, anticancer, and antihyperlipidemic effects. In this study, we explored the molecular mechanism of its immunostimulatory potency in immune responses of macrophages, using exopolysaccharides prepared from liquid culture of Lentinus edodes. We found that fraction II (F-II), with large molecular weight protein polysaccharides, is able to strongly upregulate the phenotypic functions of macrophages such as phagocytic uptake, ROS/NO production, cytokine expression, and morphological changes. F-II triggered the nuclear translocation of NF-κB and activated its upstream signaling cascades such as PI3K/ Akt and MAPK pathways, as assessed by their phosphorylation levels. The function-blocking antibodies to dectin-1 and TLR-2, but not CR3, markedly suppressed F-II-mediated NO production. Therefore, our data suggest that mushroomderived β-glucan may exert its immunostimulating potency via activation of multiple signaling pathways.
|
|
|
KEYWORD
|
|
|
Mushroom-derived β-glucan, Lentinus edodes, macrophage functions, pattern-recognition receptors, signaling cascade
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|