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KMID : 0545120080180101683
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 10 p.1683 ~ p.1688
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Antiinflammatory Effect of Lactic Acid Bacteria: Inhibition of Cyclooxygenase-2 by Suppressing Nuclear Factor-κB in Raw264.7 Macrophage Cells
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Lee Jeong-Min
Hwang Kwon-Tack
Jun Woo-Jin
Park Chang-Soo
Lee Myung-Yul
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Abstract
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Lactobacillus casei 3260 (L. casei 3260) was evaluated in relation to the inflammatory response mediated by lipopolysaccharide (LPS)-induced nuclear factor-κB (NF-κB) and cyclooxygenase-2 (COX-2) expression in Raw264.7 macrophage cells. The treatment of Raw264.7 cells with L. casei 3260 significantly inhibited the secretion of tumor necrosis factor-α (TNF-α) and prostaglandins E2 (PGE2), followed by suppression of COX-2. To clarify the molecular mechanism, the inhibitory effect of L. casei 3260 on the NF-κB signaling pathway was examined based on the luciferase reporter activity. Although the treatment of Raw264.7 cells with L. casei 3260 did not affect the transcriptional activity of NF-κB, it did inhibit NF-κB activation, as determined by the cytosolic p65 release and degradation of I-κBα. Therefore, these findings suggest that the suppression of COX-2 through inhibiting the NF-κB activation by LPS may be associated with the antiinflammatory effects of L. casei 3260 on Raw264.7 cells.
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KEYWORD
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L. casei 3260, NF-κB, COX-2, TNF-α, Raw264.7 cells
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