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KMID : 0545120090190090951
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 9 p.951 ~ p.959
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Study of Macrophage Activation and Structural Characteristics of Purified Polysaccharides from the Fruiting Body of Hericium erinaceus
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Lee Jong-Seok
Cho Jae-Youl
Hong Eock-Kee
Min Kyoung-Min
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Abstract
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Most, if not all, Basidiomycetes mushrooms have biologically active polysaccharides showing potent antitumor activity with immunomodulating properties. These polysaccharides have various chemical compositions and belong primarily to the ￥a-glucan group. In this study, the crude water-soluble polysaccharide HEF-P, which was obtained from the fruiting body of Hericium erinaceus by hot water extraction and ethanol precipitation, was fractionated by DEAEcellulose and Sepharose CL-6B column chromatographies. This process resulted in four polysaccharide fractions, named HEF-NP Fr I, HEF-NP Fr II, HEF-AP Fr I, and HEF-AP Fr II. Of these fractions, HEF-AP Fr II was able to upregulate the functional events mediated by activated macrophages, such as production of nitric oxide and expression of cytokines (IL-1￥a and TNF-￥a). The molecular mass of HEF-AP Fr II was estimated by gel filtration to be 13 kDa. Its structural characteristics were investigated by a combination of chemical and instrumental analyses, including methylation, reductive cleavage, acetylation, Fourier transform infrared spectroscopy (FT-IR), and gas chromatography-mass spectrometry (GC-MS). Results indicate that HEF-AP Fr II is a low-molecular-mass polysaccharide with a laminarin-like triple helix conformation of a ￥a-1,3-branched-￥a-1,6-glucan.
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KEYWORD
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Hericium erinaceus, immunostimulating polysaccharide, mushroom-derived β-glucan, macrophage activation, laminarin-like triple helix
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