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KMID : 0545120110210020170
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 2 p.170 ~ p.175
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Transcriptional Analysis for Oral Vaccination of Recombinant Viral Proteins against White Spot Syndrome Virus (WSSV) in Litopenaeus vannamei
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Choi Mi-Ran
Kim Yeong-Jin
Jang Ji-Suk
Kim Sung-Koo
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Abstract
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This study was carried out for the molecular level identification of recombinant protein vaccine efficacy, by oral feeding against white spot syndrome virus infection, with the comparison of viral mRNA transcriptional levels in shrimp cells. For the determination of WSSV dilution ratio for the vaccination experiment by oral feeding, in vivo virus titration was carried out using different virus dilutions of virus stock (1×102, 2×102, and 1×103). Among the dilution ratios, 2×102 diluted WSSV stock was chosen as the optimal condition because this dilution showed 90% mortality at 10 days after virus injection. Recombinant viral proteins, rVP19 and rVP28, produced as protein vaccines were delivered in shrimps by oral feeding. The cumulative mortalities of the shrimps vaccinated with rVP19 and rVP28 at 21 days after the challenge with WSSV were 66.7% and 41.7%, respectively. This indicates that rVP28 showed a better protective effect against WSSV in shrimp than rVP19. Through the comparison of mRNA transcriptional levels of viral genes from collected shrimp organ samples, it was confirmed that viral gene transcriptions of vaccinated shrimps were delayed for 4~10 days compared with those of unvaccinated shrimps. Protection from WSSV infection in shrimp by the vaccination with recombinant viral proteins could be accomplished by the prevention of entry of WSSV due to the shrimp immune system activated by recombinant protein vaccines.
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KEYWORD
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Litopenaeus vannamei, protein vaccine, WSSV, RT-PCR, viral protein
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