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KMID : 0545120110210050536
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 5 p.536 ~ p.539
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Colorimetric Evaluation of the Time-Killing Assay for Citropin 1.1, Lipopeptide Palm-KK-NH2, and Temporin A
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Wioletta Baranska-Rybak
Malgorzata Dawgul
Sylwia Bielinska
Bartlomiej Kraska
Lidia Piechowicz
Wojciech Kamysz
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Abstract
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Nowadays, there are a number of colorimetric techniques available for the determination of a time killing assay in a manner much easier and faster than those previously more commonly used, which were much more time-consuming and laborious colony counting procedures. Here, an attempt has been made to test the antimicrobial peptides of Citropin 1.1, Palm-KK-NH2, and Temporin A on a reference strain of Staphylococcus aureus using resazurin as the cell viability reagent. Staphylococcus aureus was exposed to the test compounds over varying periods of time and the metabolic activity measured, with a profile of antimicrobial activity then established. The results are in agreement with data from previous literature, thus confirming the relevance of the application of resazurin for the testing of antimicrobial agents.
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KEYWORD
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time-killing assay, Staphylococcus aureus, resazurin, antimicrobial peptides
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