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KMID : 0545120120220101401
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 10 p.1401 ~ p.1405
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Construction of a Thermotolerant Saccharomyces cerevisiae Strain for Bioethanol Production with Reduced Fermentation Time and Saccharifying Enzyme Dose
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Lim Ji-Sung
Jang You-Ri
Lim Young-Hoon
Kim Keun
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Abstract
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A thermotolerant Saccharomyces cerevisiae mutant strain, TT6, was constructed after multi-parental hybridization of five mutant strains obtained by UV or NTG treatment of the original strain, S. cerevisiae KV1. When incubated at 40oC in YPD broth, TT6 began to grow exponentially in 10 h, but KV1 did not show any noticeable growth even after 22 h. The thermotolerant growth of TT6 was confirmed by serial dilution assay at 42oC; TT6 grew at a cell concentration (10-5) 10,000 times lower than that of KV1 (10-1). Whereas ethanol production from YP containing 23% (w/v) glucose by KV1 decreased with increasing temperature from 30oC to 36oC, ethanol production by TT6 did not decrease at temperatures up to 37oC. When TT6 was tested for ethanol production at 36oC by simultaneous saccharification and fermentation (SSF) from 23% corn, 24 h of fermentation time or 50% of the glucoamylase dose was saved when compared with KV1 at 30oC. The ethanol yield from corn by SSF with TT6 at 36oC was 91.7% of the theoretical yield, whereas that of KV1 at 30oC was 90.6%.
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KEYWORD
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Thermotolerance, Saccharomyces cerevisiae, bioethanol, growth, fermentation time, glucoamylase
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