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KMID : 0545120160260010072
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Journal of Microbiology and Biotechnology
2016 Volume.26 No. 1 p.72 ~ p.79
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Asymmetric Bioconversion of Acetophenone in Nano-Sized Emulsion Using Rhizopus oryzae
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Li Qing Zhi
Shi Yang
He Le
Zhao Hui
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Abstract
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The fungal morphologies and pellet sizes were controlled in acetophenone reduction by Rhizopus oryzae. The acetophenone conversion and (S)-phenylethanol enantiomeric excesses (e.e.) reached the peak after 72 h of incubation when using pellets with 0.54 mm diameter, which showed an excellent performance compared with suspended mycelia, clumps, and pellets with 0.65 or 0.75 mm diameter. Furthermore, nano-sized acetophenone was used as a substrate to improve the performances of biotransformation work. The results showed that the conversion of nanometric acetophenone and (S)-phenylethanol e.e. reached the maximum (both >99%) after 32 h of incubation when using 0.54 mm diameter pellets, at least 24 h in advance of the control group. On the other hand, Tween 80 and 1, 2-propylene glycol showed low or no toxicity to cells. In conclusion, pellets and acetophenone nanoemulsions synergistically result in superior performances of acetophenone reduction.
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KEYWORD
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Acetophenone, Bioreduction, Conversion, Enantiomeric excesses (e.e.), Nanoemulsion, Rhizopus oryzae
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