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KMID : 0545120160260071216
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Journal of Microbiology and Biotechnology
2016 Volume.26 No. 7 p.1216 ~ p.1223
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Purification and Identification of Antioxidant Peptides from Enzymatic Hydrolysate of Spirulina platensis
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Yu Jie
Hu Yuan Liang
Xue Ming Xiong
Dun Yao Hao
Li She Nao
Peng Nan
Liang Yun Xiang
Zhao Shu Mao
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Abstract
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The aim of this study was to isolate antioxidant peptides from an enzymatic hydrolysate of Spirulina platensis. A novel antioxidant peptide was obtained by ultrafiltration, gel filtration chromatography, and reverse-phase high-performance liquid chromatography, with the 1,1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay used to measure the antioxidant activity, and the sequence was determined to be Pro-Asn-Asn (343.15 Da) by electrospray ionization tandem mass spectrometry. This peptide was synthesized to confirm its antioxidant properties, and it exhibited 81.44 ± 0.43% DPPH scavenging activity at 100 μg/ml, which was similar to that of glutathione (82.63 ± 0.56%). Furthermore, the superoxide anion and hydroxyl free-radical scavenging activities and the SOD activity of the peptide were 47.84 ± 0.49%, 54.01 ± 0.82%, and 12.55 ± 0.75%, respectively, at 10 mg/ml. These results indicate that S. platensis is a good source of antioxidant peptides, and that its hydrolysate may have important applications in the pharmaceutical and food industries.
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KEYWORD
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Antioxidant peptide, free radical-scavenging activity, mass spectrometry, purification, Spirulina platensis
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