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KMID : 0545120170270091559
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 9 p.1559 ~ p.1565
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Gram-Scale Production of Ginsenoside F1 Using a Recombinant Bacterial β-Glucosidase
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An Dong-Shan
Cui Chang-Hao
Siddiqi Muhammad Zubair
Yu Hong Shan
Jin Feng-Xie
Kim Song-Gun
Im Wan-Taek
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Abstract
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Naturally occurring ginsenoside F1 (20-O-β-D-glucopyranosyl-20(S)-protopanaxatriol) is rare. Here, we produced gram-scale quantities of ginsenoside F1 from a crude protopanaxatriol saponin mixture comprised mainly of Re and Rg1 through enzyme-mediated biotransformation using recombinant β-glucosidase (BgpA) cloned from a soil bacterium, Terrabacter ginsenosidimutans Gsoil 3082T. In a systematic step-by-step process, the concentrations of substrate, enzyme, and NaCl were determined for maximal production of F1. At an optimized NaCl concentration of 200 mM, the protopanaxatriol saponin mixture (25 mg/ml) was incubated with recombinant BgpA (20 mg/ml) for 3 days in a 2.4 L reaction. Following octadecylsilyl silica gel column chromatography, 9.6 g of F1 was obtained from 60 g of substrate mixture at 95% purity, as assessed by chromatography. These results represent the first report of gramscale F1 production via recombinant enzyme-mediated biotransformation.
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KEYWORD
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Protopanaxatriol, bioconversion, gram-scale, glycoside hydrolase family 3, rare ginsenoside
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