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KMID : 0545120180280020293
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 2 p.293 ~ p.297
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Enhancement of L-Threonine Production by Controlling Sequential Carbon-Nitrogen Ratios during Fermentation
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Lee Hyeok-Won
Lee Hee-Suk
Kim Chun-Suk
Lee Jin-Gyeom
Kim Won-Kyo
Lee Eun-Gyo
Lee Hong-Weon
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Abstract
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Controlling the residual glucose concentration is important for improving productivity in L-threonine fermentation. In this study, we developed a procedure to automatically control the feeding quantity of glucose solution as a function of ammonia-water consumption rate. The feeding ratio (RC/N) of glucose and ammonia water was predetermined via a stoichiometric approach, on the basis of glucose-ammonia water consumption rates. In a 5-L fermenter, 102 g/l L-threonine was obtained using our glucose-ammonia water combined feeding strategy, which was then successfully applied in a 500-L fermenter (89 g/l). Therefore, we conclude that an automatic combination feeding strategy is suitable for improving L-threonine production.
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KEYWORD
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L-threonine, feeding ratio (Rc/n), feeding strategy, residual glucose concentration, scale-up
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