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KMID : 0545120180280030349
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 3 p.349 ~ p.356
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Immune Enhancement Effect of Asterias amurensis Fatty Acids through NF-κB and MAPK Pathways on RAW 264.7 Cells
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Monmai Chaiwat
Go Seok-Hyeon
Shin Il-Shik
You Sang-Guan
Lee Hyung-Jae
Kang Seok-Beom
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Abstract
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Asterias amurensis is a marine organism that causes damage to the fishing industry worldwide; however, it has been considered a promising source of functional components. The present study aimed to investigate the immune-enhancing effects of fatty acids from three organs of A. amurensis on murine macrophages (RAW 264.7 cells). A. amurensis fatty acids boosted production of immune-associated factors such as nitric oxide (NO) and prostaglandin E2 in RAW 264.7 cells. A. amurensis fatty acids also enhanced the expression of critical immuneassociated genes, including iNOS, TNF-α, IL-1β, and IL-6, as well as COX-2. Western blotting showed that A. amurensis fatty acids stimulated the NF-κB and MAPK pathways by phosphorylation of NF-κB p-65, p38, ERK1/2, and JNK. A. amurensis fatty acids from different tissues resulted in different levels of NF-κB and MAPK phosphorylation in RAW 264.7 cells. The results increase our understanding of how A. amurensis fatty acids boost immunity in a physiological system, as a potential functional material.
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KEYWORD
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Immune enhancement, fatty acid, NF-κB pathway, MAPK pathway, starfish
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