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KMID : 0545120180280040579
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 4 p.579 ~ p.587
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Hanseniaspora thailandica BC9 β-Glucosidase for the Production of β-D-Hexyl Glucoside
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Phongprathet Sujittra
Vichitphan Kanit
Han Jae-Hong
Vichitphan Sukanda
Sawaengkaew Jutaporn
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Abstract
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For biotechnological production of high-valued β-D-hexyl glucoside, the catalytic properties of Hanseniaspora thailandica BC9 β-glucosidase purified from the periplasmic fraction were studied, and the transglycosylation activity for the production of β-D-hexyl glucoside was optimized. The constitutive BC9 β-glucosidase exhibited maximum specific activity at pH 6.0 and 40ºC, and the activity of BC9 β-glucosidase was not significantly inhibited by various metal ions. BC9 β-glucosidase did not show a significant activity of cellobiose hydrolysis, but the activity was rather enhanced in the presence of sucrose and medium-chain alcohols. BC9 β-glucosidase exhibited enhanced production of β-D-hexyl glucoside in the presence of DMSO, and 62% of β-D-hexyl glucoside conversion was recorded in 4 h in the presence of 5% 1-hexanol and 15% DMSO.
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KEYWORD
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Hanseniaspora thailandica BC9, β-glucosidase, transglycosylation, alkyl glycoside, β-D-hexyl glucoside
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