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KMID : 0545120180280040652
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 4 p.652 ~ p.662
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Comparative Analysis of Gut Microbial Communities in Children under 5 Years Old with Diarrhea
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Wen Hongyu
Yin Xin
Yuan Zhenya
Wang Xiuying
Su Siting
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Abstract
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Diarrhea is a global disease with a high morbidity and mortality rate in children. In this study, 25 fecal samples were collected from children under 5 years old. Seven samples had been taken from healthy children without diarrhea and marked as the healthy control group; eight samples had been sampled from children with diarrhea caused by dyspepsia and defined as the non-infectious group; and ten samples had been taken from children with diarrhea induced by intestinal infections and identified as the infectious group. We detected the microbial communities of samples by using high-throughput sequencing of 16S rRNA genes. The proportion of aerobic and facultative anaerobic microbes in samples of the infectious group was much higher than in the non-infectious group. In addition, the relative abundance of Enterococcus in the healthy control group was significantly higher than in the non-infectious group and infectious group. This can be used as a potential diagnostic biomarker for diarrhea.
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KEYWORD
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Children diarrhea, gut microbial communities, high-throughput sequencing, 16S rRNA genes
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