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KMID : 0578320190420020175
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Molecules and Cells
2019 Volume.42 No. 2 p.175 ~ p.182
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The MicroRNA-551a/MEF2C Axis Regulates the Survival and Sphere Formation of Cancer Cells in Response to 5-Fluorouracil
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Kang Ho-In
Kim Chong-Tae
Ji Eun-Byul
Ahn So-Jin
Jung Myeong-Woo
Hong You-Lim
Kim Wook
Lee Eun-Kyung
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Abstract
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microRNAs regulate a diverse spectrum of cancer biology, including tumorigenesis, metastasis, stemness, and drug resistance. To investigate miRNA-mediated regulation of drug resistance, we characterized the resistant cell lines to 5-fluorouracil by inducing stable expression of miRNAs using lenti-miRNA library. Here, we demonstrate miR-551a as a novel factor regulating cell survival after 5-FU treatment. miR-551a-expressing cells (Hep3B-lenti-miR-551a) were resistant to 5-FU-induced cell death, and after 5-FU treatment, and showed significant increases in cell viability, cell survival, and sphere formation. It was further shown that myocyte-specific factor 2C is the direct target of miR-551a. Our results suggest that miR-551a plays a novel function in regulating 5-FU-induced cell death, and targeting miR-551a might be helpful to sensitize cells to anti-cancer drugs.
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KEYWORD
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anti-cancer drug resistance, cell survival, hepatocellular carcinoma, microRNA, sphere formation
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